WebAkt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor ... WebAnti-phospho-Akt (pThr308); Anti-phospho-Akt (pThr308) antibody produced in rabbit has been used in immunoblotting. US EN. Applications Products Services Support. …
Akt/PKB signaling pathway - Wikipedia
WebThe Phospho-AKT (Thr308) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the … WebMar 22, 2024 · The protein levels of p-Akt (S473 and T308), Akt, p-rpS6 (S235/236, S240/244), rpS6, FLAG and β-actin were detected with immunoblotting. e HEK293T cells were transfected with FLAG empty vector or FLAG-DRAM1 for 24 h, and then starved for another 18 h before stimulated with IGF-1, EGF or serum (10%) for 10 min in the presence … order notary supplies nj
Phospho-AKT1 (Thr308) Antibody (44-602G)
WebMore>> Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA. Less<< MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. WebPrimary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min ... View more Product Details Product Specific Information Store vial at 4° C prior to restoration. WebThe Phospho-Akt (Thr308) Antibody was generated using AKT1, AKT2, and AKT3 as the antigen and it reacts with Human, Mouse, and Rat. Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308 Phospho-Akt (Thr308) Antibody Supplier Page Product Specs Company Info Citations Reviews Product Specs ireland to myt